Open AccessDetection of gene expression in sentinel lymph node of primary breast cancer patients
Author(s) -
Shahrzad Soleimani,
Shohreh Alizadeh Shargh,
Aliasghar Keramatinia,
Narjes Mehrvar,
Neda Mansouri,
Abbas Doosti,
Seyed Abdolreza Mortazavi-Tabatabaei,
Mehrdad Hashemi,
Abolfazl Movafagh
Publication year - 2018
Publication title -
cellular and molecular biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.371
H-Index - 71
eISSN - 1165-158X
pISSN - 0145-5680
DOI - 10.14715/cmb/2018.64.5.20
Subject(s) - breast cancer , oncology , medicine , stage (stratigraphy) , primary tumor , lymph node , gene signature , metastasis , gene expression , gene , cancer , logistic regression , pathology , biology , paleontology , biochemistry
Sentinel lymph node (SLN) micrometstasis detection improves outcome for breast cancer follow up procedure. The aim of the present study was to identify gene profiles that accurately predicted the outcome of breast cancer patients. Fifty tumor sample from breast cancer patients were analyzed for the expression of 3 genes using quantitative-PCR. Also clinical verification for recurrence to distant organs was performed. Three gene signature were confirmed based on tumor's stage, grade, ER status, using conditional logistic regression. Based on this findings, the negative reported lymph nodes for metastasis, had micro metastasis in significant values. There was a significant difference between normal and cancer samples in 3 gene expression marker and also there was meaningful relationship between three gene expression with tumor's grade, stage according to progression of tumor. A novel gene expression signature predictive of micro metastatic patients was evaluated. In this assessment, relationship between this gene with tumor's features that finding clear role for these genes with tumor's outcome, needs to be established.