Open AccessN-Acetylglucosamine Production by Repeated-Batch Fermentation Using Immobilized Semi-Purified Chitinase Enzyme on Agar
Author(s) -
Lucia Crysanthy Soedirga,
Hardoko Hardoko,
Natasha Vania Widianto
Publication year - 2021
Publication title -
reaktor
Language(s) - English
Resource type - Journals
eISSN - 2407-5973
pISSN - 0852-0798
DOI - 10.14710/reaktor.21.1.11-14
Subject(s) - chitinase , fermentation , chitin , agar , hydrolysis , agar plate , enzyme , food science , biochemistry , chemistry , chromatography , biology , bacteria , chitosan , genetics
Chitinolytic mold, such as Mucor circinelloidesis can be utilized to produce chitinase enzyme for shrimp shell’s chitin hydrolysis into N-acetylglucosamine (NAG). For that purpose, entrapment of chitinase on agar as a carrier could be an alternative way to improve NAG production. This study aimed to investigate the stability of immobilized semi-purified chitinase on agar for multiple cycles fermentation to produce NAG. In this study, 0.6 mL of semi-purified chitinase enzyme was immobilized into 3% of agar matrices and tested for four fermentation cycles to obtain highest NAG concentration and good enzyme activity. The results indicate that the immobilized chitinase could be used for 6 hours fermentation or three fermentation cycles. The NAG concentration produced after three cycle were 1042.22 ± 16.20 ppm. Besides, the immobilized enzyme was considerably stable up to the third cycles with activity value of about 4.74 U/mL.Keywords: agar; immobilized;NAG; repeated fermentation