Open AccessImmobilization of Crude Polyphenol Oxidase Extracts from Apples on Polypyrrole as a Membrane for Phenol Removal
Author(s) -
Anceu Murniati,
Buchari Buchari,
Suryo Gandasasmita,
Zeily Nurachman,
Arie Hardian,
Dera Triani
Publication year - 2021
Publication title -
jurnal kimia sains dan aplikasi/jurnal kimia sains dan aplikasi
Language(s) - English
Resource type - Journals
eISSN - 2597-9914
pISSN - 1410-8917
DOI - 10.14710/jksa.24.2.62-69
Subject(s) - polyphenol oxidase , chemistry , polypyrrole , membrane , phenol , substrate (aquarium) , chromatography , anode , filtration (mathematics) , nuclear chemistry , browning , electrolyte , polyphenol , electrode , food science , enzyme , organic chemistry , biochemistry , antioxidant , electrochemistry , peroxidase , oceanography , statistics , mathematics , geology
This research aims to make a polypyrrole (PPy) membrane and crude extract of polyphenol oxidase (PPO) as a membrane of mPPy/PPO apple extracts. The membrane of PPy/PPO-apple extract has been synthesized by the electrodeposition method. The electrolyte composition consists of a mixture of 0.10-0.20 M pyrrole (Py) and 50-100% PPO apple extract, which is stable using 50 mM of phosphate buffer solution at pH 6.80-7.00 and room temperature. The electrodeposition process is used 400 mesh steel gauze anode ST-304 and carbon plate cathode. Electrodeposition is carried out at potential = 5.00-6.00 V; current = 0.02-0.25 A; the distance from both electrodes = 1.00-2.00 cm for 300-500 seconds. The results from the deposition of PPy/PPO apple extract of the anode are a membrane of mPPy/PPO-apple extract, with total enzyme activity (U) = (957,1441, 2287 and 1754) using 2.00-5.00 mM phenol as a substrate which is measured based on the UV-visible spectrophotometric method. PPy and mPPy/PPO-apple extracts were characterized by SEM and SEM-EDS. The membrane of mPPy/PPO-apple extract can be used to remove phenol in industrial wastewater samples is 50-65% with a filtration capacity of 500 mL for 2 hours.