Open AccessOptimization of Lipase Production from Local Bacteria Isolate with Palm Oil Inducer
Author(s) -
Dwi May Indriyani,
Nurhasanah Nurhasanah,
Dian Herasari
Publication year - 2021
Publication title -
jurnal kimia sains dan aplikasi/jurnal kimia sains dan aplikasi
Language(s) - English
Resource type - Journals
eISSN - 2597-9914
pISSN - 1410-8917
DOI - 10.14710/jksa.24.2.58-61
Subject(s) - lipase , inducer , chemistry , food science , palm oil , hydrolysis , interesterified fat , triacylglycerol lipase , biochemistry , incubation , enzyme , gene
A lipase is a group of hydrolase enzymes with multi-functional abilities such as catalyzing hydrolysis, alcoholysis, esterification, interesterification, and synthesis of organic compounds. The purpose of this study was to obtain the optimum conditions for lipase production and to study the effect of adding a palm oil inducer on the production of lipase from local bacterial isolates. The method used in this study included the rejuvenation of local bacterial isolate cultures, optimization of production, which included pH, palm oil inducer, and incubation time. The lipase activity test was carried out by using the acid-base titration method with the phenolphthalein indicator. The results showed that the optimal conditions for lipase production occurred at pH 5 with an activity value of 7.5 U/mL and an incubation time of 18 hours with an activity value of 13.83 U/mL. The addition of a 10% (v/v) palm oil inducer increased the value of lipase activity to 8.75 U/mL.