Open AccessOptimasi Isolasi DNA Cabai (Capsicum annuum L.) Berdasar Perbedaan Kualitas dan Kuantitas Daun serta Teknik Penggerusan
Author(s) -
Rejeki Siti Ferniah,
Sri Pujiyanto
Publication year - 2013
Publication title -
bioma : berkala ilmiah biologi/bioma
Language(s) - English
Resource type - Journals
eISSN - 2598-2370
pISSN - 1410-8801
DOI - 10.14710/bioma.15.1.14-19
Subject(s) - capsicum annuum , grinding , dna , agarose gel electrophoresis , isolation (microbiology) , dna extraction , chemistry , biology , horticulture , materials science , polymerase chain reaction , microbiology and biotechnology , biochemistry , gene , pepper , composite material
Complete genome of chili has not been reported.The first step to study the genome is DNA isolation, so it is necessary to optimize the protocol to get an optimum DNA. This research aimed to optimize chili DNA isolation by variate the quantity and quality of chili leaves as row material and variate the grinding technique. DNA isolation was done using commercial kit without liquid nitrogen, and analyzed by agarose gel electrophoresis. The results showed that frozen chili leaf yields more DNA than fresh leaf, 0,1 g of leaf got optimum DNA, and grinding in mortar was better than in microtube. Key words: DNA, isolation, Capsicum annuum