Open AccessEthanolic Extract of Hedyotis corymbosa L. Inhibits Migration and MMP-9 Activity on Metastatic Breast Cancer Cells
Author(s) -
Dhania Novitasari,
Sri Handayani,
Riris Istighfari Jenie
Publication year - 2018
Publication title -
iscc (indonesian journal of cancer chemoprevention)
Language(s) - English
Resource type - Journals
eISSN - 2355-8989
pISSN - 2088-0197
DOI - 10.14499/indonesianjcanchemoprev9iss1pp16-22
Subject(s) - traditional medicine , cytotoxicity , metastasis , breast cancer , chemistry , ic50 , mtt assay , cancer cell , cytotoxic t cell , cancer , pharmacology , in vitro , biology , medicine , biochemistry
Many natural products have been widely explored for their pharmacological activities, including anticancer activity. Hedyotis corymbosa L. is known for its anticancer properties toward several cancer cell lines. The study aims to investigate whether ethanolic extract of Hedyotis corymbosa L. (EEH) performs anticancer properties by inhibiting migration and metastasis on breast cancer cells. Cytotoxic evaluation by using MTT assay was carried out to determine EEH effect on 4T1 breast cancer cells, meanwhile to investigate the treatment of EEH in migration and metastasis inhibitory effect, scratch wound healing assay and gelatin zymography were conducted in this study. The data showed that EEH possessed cytotoxic activity with IC50 value of 400 μg/mL. Interestingly, migration inhibitory effect was shown up to 42 hours and the activity of MMP-9 was also decreased after the treatment with EEH. According to these findings, we suggest that Hedyotis corymbosa L. promotes another anticancer properties by inhibiting migration and metastasis towards breast cancer cells.Keywords : Hedyotis corymbosa L., cytotoxicity, migration, metastatic, 4T1 breast cancer cells