Open AccessPO-161 Effect of different type of exercise on mitochondrial homeostasis in rats with myocardial infarction
Author(s) -
Hai Bai,
Yong Zhang
Publication year - 2018
Publication title -
exercise biochemistry review
Language(s) - English
Resource type - Journals
ISSN - 2593-7588
DOI - 10.14428/ebr.v1i4.11883
Subject(s) - tfam , myocardial infarction , medicine , cardiology , mfn2 , pink1 , endocrinology , mitochondrion , mitochondrial fusion , mitochondrial dna , biology , parkin , biochemistry , disease , mitochondrial biogenesis , gene , parkinson's disease , microbiology and biotechnology
Objective To investigate the different effect of moderate-intensity continuous training (MCT) and high-intensity interval exercise training (HIT) on ventricular remodeling and mitochondrial homeostasis after acute myocardial infarction (AMI).
Methods The AMI rat model was achieved by ligating coronary artery. The AMI and sham operation rats were randomly divided into four groups: sham operation group (Sham), AMI control group (AMI), AMI MCT group (AMI+M), and AMI HIT group (AMI+H). Animals in the AMI+M and AMI+H groups underwent 4 weeks MCT and HIT respectively. Five weeks after AMI, hemodynamic changes, mitochondrial bioenergetics, and PINK1, Beclin1, Mfn2, Drp1, Tfam, COXⅣ, PGC-1α were detected.
Results Comparing with AMI group, in AMI+M and AMI+H groups, Beclin1 (146.33±18.47, 143.28±16.96.vs. 123.27±13.27, P<0.05), PINK1 (150.33±20.54, 152.28±18.34.vs. 125.27±17.67, P<0.05), Mfn2 (122.28±18.81, 117.19±17.04.vs. 46.27±6.72, P<0.01), and PGC-1α (82.15±16.58, 102.25±13.27.vs. 60.27±9.36, P<0.05~0.01) expression elevated significantly, whereas ROS generation (122.28±18.81, 117.19±17.04.vs. 46.27±6.72, P<0.01) and Drp1 expression (9.58±1.40, 10.18±1.37.vs. 15.85±1.61, P<0.05) showed dramatic decrease. In addition, in AMI+H group, +dp/dt max (6326±325.vs. 5368±271, P<0.05), -dp/dt max (-5312±246.vs. -4457±250, P<0.05), mitochondrial membrane potential (85.24±11.94.vs. 71.28±8.34, P<0.05), ATP synthesis activity (38.77±5.16.vs. 32.33±4.14, P<0.05), Tfam (95.25±12.05.vs. 78.27±12.22, P<0.05) and COXⅣ (89.25±14.06.vs. 67.27±13.71, P<0.05) expression improved significantly. Comparing with AMI+M group, in AMI+H group, +dp/dt max (6326±325.vs. 5775±310, P<0.05), -dp/dt max (-5312±246.vs. -4778±305, P<0.05), PGC-1α (102.25±13.27.vs. 82.15±16.58 1, P<0.05), Tfam (95.25±12.05.vs. 80.15±11.99, P<0.05) and COXⅣ(89.25±14.06.vs. 72.15±10.89, P<0.05) expression improved significantly.
Conclusions High-intensity interval exercise training is superior to moderate-intensity continuous training for ameliorating ventricular remodeling and mitochondrial homeostasis after acute myocardial infarction.