Open AccessDetection of Porcine Adulteration in Cosmetic Cream Formulation via TaqMan Probe Real-Time Polymerase Chain Reaction.
Author(s) -
S S Abd-Gani,
Shuhaimi Mustafa,
Mohd Nasir Mohd Desa,
Nur Fadhilah Khairil Mokhtar,
Ummi Kalthum Hanapi,
Zalina Zakaria,
Nazariyah Yahaya,
W M A Wan Sulaiman
Publication year - 2019
Publication title -
international journal of engineering and technology
Language(s) - English
Resource type - Journals
ISSN - 2227-524X
DOI - 10.14419/ijet.v7i4.14.27509
Subject(s) - taqman , amplicon , polymerase chain reaction , 18s ribosomal rna , real time polymerase chain reaction , chemistry , food science , microbiology and biotechnology , chromatography , biology , ribosomal rna , gene , biochemistry
The identification of lard adulterated in cosmetic cream using TaqMan probe real-time Polymerase Chain Reaction was developed in this research. The laboratory prepared cream was formulated with oil in water (o/w) emulsion with the adulteration of 1%, 3% and 5% of lard (w/w). The total DNA from lard-adulterated cosmetic cream was successfully extracted. The detection assay targeting different gene; universal 18S rRNA and porcine-specific multiple porcine repetitive element (MPRE) which employed with the TaqMan probe. This study targeting 187 bp of amplicons for 18S rRNA while 99 bp of amplicons for Porcine-specific probes. A comparison of quantification cycle (Cq) with prior to percentage of adulteration were detected. Lard-adulterated cream was amplified for 18S rRNA while no detection was detected for Porcine-specific probes. The Cq value obtained might be vary as proposed by the hypothesis depending on the total recovery of extracted DNA from the lard-adulterated cream.