Open AccessAnti-Acetylcholinesterase, Anti-Inflammatory and Anti-Oxidant Activities of Raw-Extract Centella Asiatica (RECA) on Lipopolysaccharide (LPS)-Induced Neuroinflammation Sprague Dawley Rats
Author(s) -
Zetty Zulikha Hafiz,
Nursyamila Shamsuddin,
Shahida Muhamad Mukhtar,
R J James,
Mohd Ilham Adenan
Publication year - 2019
Publication title -
international journal of engineering and technology
Language(s) - English
Resource type - Journals
ISSN - 2227-524X
DOI - 10.14419/ijet.v7i4.14.27479
Subject(s) - centella , acetylcholinesterase , aché , lipopolysaccharide , oxidative stress , pharmacology , in vivo , chemistry , neuroinflammation , ic50 , traditional medicine , in vitro , inflammation , biology , medicine , biochemistry , enzyme , immunology , microbiology and biotechnology
The present study was designed to investigate the potential of raw-extract of Centella asiatica (RECA) in suppressing acetylcholinesterase (AChE), inflammations and oxidative stress via induction of lipopolysaccharide (LPS) on animal model of Sprague Dawley rats. Centella asiatica is a plant that has been used as a traditional herbal remedy for the management of several diseases, including memory improvement, treatment of mental fatigue and wound healing. Pre-treatment with RECA in vitro significantly reduced the AChE activity in a concentration-dependent manner with IC50 value of 57.47 ± 13.55 µg/ml. Interestingly, this result was parallel with in vivo studies. Moreover, the level of pro-inflammatory cytokines and oxidative stress were significantly reduced by RECA in dose-dependent manner. Overall, our findings clearly dictate the potential of RECA as AChE inhibitor as well anti-inflammatory and anti-oxidant agents.