Open AccessToxicity Analysis on Weight-Loss Supplement using Allium Test and Simple Sequence Repeat (SSR)
Author(s) -
Norfatimah Mohamed Yunus,
Lateef Ahmad,
I Mahadi,
M. S. Yahaya
Publication year - 2019
Publication title -
international journal of engineering and technology
Language(s) - English
Resource type - Journals
ISSN - 2227-524X
DOI - 10.14419/ijet.v7i4.14.27469
Subject(s) - allium , toxicity , mitotic index , toxicology , weight loss , biology , traditional medicine , microbiology and biotechnology , mitosis , genetics , botany , medicine , obesity , endocrinology
Recently, consumptions of weight-loss supplement to reduce and maintain body weight had tremendously increased among local people. The effect of this supplement on dividing cells particularly on chromosome and DNA should be addressed due to lack of information by manufacturers regarding the ingredients. This study was carried out to observe any toxicity effects of two commercial weight-loss supplements through Allium test and simple sequence repeat (SSR). Toxicity analysis was investigated through observation on mitotic cells of Allium cepa which includes mitotic index and chromosomes analyses at 24 and 48 hours of treatments. The genotoxic effects of brand X at 24 hours was identified by SSR based on different sizes of band (230 bp) compared to control (200 bp). At 48 hours, positive genotoxic effects were observed through chromosomes and DNA analyses. Meanwhile, brand Y showed genotoxic effect at 24 hours as suggested by both studies. Prolonged treatment at 48 hours proved the cytotoxic and genotoxic effects of brand Y as supported by Allium test and SSR. This preliminary finding is beneficial for future study on toxicity effects of weight-loss supplements which will help to increase public awareness on the risks of consumption without consultation of nutritionist or physician.