Open AccessIsolation and molecular characterization of Mycobacterium tuberculosis complex isolated from raw milk in some dairy farms in Egypt
Author(s) -
Heba Hussien,
Eman Mahrous
Publication year - 2016
Publication title -
international journal of basic and applied sciences
Language(s) - English
Resource type - Journals
ISSN - 2227-5053
DOI - 10.14419/ijbas.v5i2.5299
Subject(s) - mycobacterium tuberculosis complex , raw milk , tuberculosis , isolation (microbiology) , tuberculin , mycobacterium tuberculosis , biology , mycobacterium , real time polymerase chain reaction , veterinary medicine , microbiology and biotechnology , food science , medicine , pathology , biochemistry , gene
This study was conducted to detect Mycobacterium tuberculosis complex in milk in three Egyptian Governorates; El-Sharkia, El-Menoufia and El-Behera Governorates. 300 milk samples were collected from tuberculin positive cases, 18 (6.0%) were shedding Mycobacterium tuberculosis complex in their milk which detected by real time PCR. On another hand, 170 milk samples were collected from tuberculin negative cases, 5 (2.9%) were shedding Mycobacterium tuberculosis complex in their milk which detected by real time PCR. All milk samples were examined by three techniques including Microscopic examination, culture and real time PCR. Real time PCR is more rapid and accurate method than microscopic and culture method. The isolated colonies from culture were examined by Multiplex PCR to demonstrate the source of infection either human or animal source.