Open AccessPCR for detection of virulence and antibiotic resistance genes of coagulase positive staphylococcus aureus from clinical mastitis in Egypt
Author(s) -
Eman Abdeen,
Mousa Walid,
Heba Hussien,
Saher Roshdy
Publication year - 2015
Publication title -
international journal of basic and applied sciences
Language(s) - English
Resource type - Journals
ISSN - 2227-5053
DOI - 10.14419/ijbas.v4i3.4630
Subject(s) - mastitis , staphylococcus aureus , microbiology and biotechnology , coagulase , amplicon , virulence , sccmec , biology , antibiotics , antibiotic resistance , pathogen , staphylococcus , typing , virology , gene , polymerase chain reaction , methicillin resistant staphylococcus aureus , bacteria , genetics
Coagulase positive Staphylococcus aureus is the most frequently bacterial pathogen causing clinical mastitis in cattle. In this work 120 milk samples were collected from cattle suffering from clinical mastitis. The result revealed that S. aureus was 35 isolates (29.16%) and other staphylococci were 13 isolates (10.84%). The application of PCR was effectively in detection of Coa. SpA, mecA and blaZ genes of S. aureus by amplification at a single amplicon at 630 bp, 226 bp, 310 bp and 173bp, respectively. These results concluded that the detection of Coa and SpA genes proposed as good method for typing of S. aureus isolates as well as detection of antibiotics resistance mecA and blaZ genes helping effective control of staphylococcal mastitis.