Open AccessDevelopment and validation of HPTLC method for the estimation of cefadroxil in bulk and tablet dosage form
Author(s) -
S V Kirankumar,
Jose Gnana Babu C,
H G Sowmya
Publication year - 2020
Publication title -
international journal of advanced chemistry
Language(s) - English
Resource type - Journals
ISSN - 2310-2977
DOI - 10.14419/ijac.v8i2.30963
Subject(s) - cefadroxil , dosage form , chromatography , silica gel , analyte , analytical chemistry (journal) , chloroform , materials science , ethyl acetate , linearity , chemistry , biochemistry , cephalosporin , antibiotics , physics , quantum mechanics
A novel and simple HPTLC method has been developed for the estimation of Cefadroxil (CFD) in bulk and pharmaceutical dosage form on silica gel precoated aluminum 60F254 plates, (10 cm x10 cm) with 0.25 mm thickness. The separation was carried out using ethyl acetate: chloroform (8:2v/v) as mobile phase. The Chromatographic scanning was carried out at 254 nm. The Rf (± SD) values were found to be 0.38 ± 0.05 for CFD. The linearity for analyte was in the range 100-500 ng/band for CFD with correlation coefficients, r2 = 0.9996. The percentage recovery obtained for CFD was in the range of 99.28-100.13% respectively. The proposed method was optimized and validated as per the ICH guidelines17.