Open AccessDevelopment and validatation of HPTLC method for estimation of irbesartan in bulk and tablet dosage form
Author(s) -
C S Yogeesh,
H G Sowmya,
Jose Gnana Babu C
Publication year - 2020
Publication title -
international journal of advanced chemistry
Language(s) - English
Resource type - Journals
ISSN - 2310-2977
DOI - 10.14419/ijac.v8i1.30800
Subject(s) - irbesartan , chromatography , silica gel , dosage form , linearity , chloroform , ethyl acetate , pharmaceutical formulation , chemistry , relative standard deviation , analytical chemistry (journal) , correlation coefficient , materials science , mathematics , detection limit , statistics , medicine , physics , alternative medicine , pathology , quantum mechanics
A simple, rapid and accurate High performance thin layer chromatography is described for the Development and validation of HPTLC method for Irbesartan in bulk and Pharmaceutical dosage form. The separation is carried out on Merck TLC aluminum sheets of silica gel 60 F254 using Ethyl acetate: Chloroform (6.5:3.5v/v) mobile phase. Quantification was done by Densitometric scanning at 254nm. The linearity was found to be the range of 100-500ng/spot for Irbesartan with the correlation coefficient of 0.9992. The regression equation was found to be Y=7.2733x+703.15. The Rf value of Irbesartan was found to be 0.55. The LOD and LOQ were found to 8.24 and 24.74 respectively. Average recovery was found to be 99.66% which show that the method was free from interference from excipients present in the formulation. Simultaneously the Percentage relative standard deviation was well within the range of 2%. The above method was validated according to the ICH guidelines. The established method enabled accurate, precise and applied to the analysis of Irbesartan in bulk and Pharmaceutical dosage form.