Open AccessPHENOTYPIC FEATURES OF T REGULATORY CELLS IN EARLY RHEUMATOID ARTHRITIS
Author(s) -
А. С. Авдеева,
Ю. П. Рубцов,
Т. В. Попкова,
Daniyar T. Dyikanov,
Е. Л. Насонов
Publication year - 2017
Publication title -
naučno-praktičeskaâ revmatologiâ
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.137
H-Index - 9
eISSN - 1995-4492
pISSN - 1995-4484
DOI - 10.14412/1995-4484-2016-660-666
Subject(s) - foxp3 , interleukin 7 receptor , il 2 receptor , cd154 , rheumatoid arthritis , medicine , flow cytometry , immunology , gastroenterology , erythrocyte sedimentation rate , biology , immune system , t cell , cd40 , in vitro , cytotoxic t cell , biochemistry
Objective: to investigate the count and characteristics of the phenotype of T regulatory cells (Treg) in the peripheral blood of healthy donors and patients with early rheumatoid arthritis (RA), by using multicolor flow cytometry. Subjects and methods . The investigation enrolled 39 patients with early RA. The percentage and absolute count of Treg (FoxP3+CD25+, surface CD152+, intracellular CD152+, FoxP3+CD127, CD25+CD127, FoxP3+ICOS+, FoxP3+CD154+; and FoxP3+CD274+) was determined by multicolor flow-cytometry. A control group consisted of 20 healthy donors matched for sex and age with the examined patients. Results and discussion. In the patients included in the study, the median [25th; 75th percentiles] DAS28 was 5.01 [4.2; 5.8]; high, moderate, and low activity showed 22 (48.9%), 20 (44.4%), and 3 (6.7%) patients, respectively. The patients with early RA had a lower percentage of FoxP3+CD25+ cells and a lower percentage and absolute count of FoxP3+ICOS+, FoxP3+CD154+, and FoxP3+CD274+ T cells than the healthy donors (p<0.05 in all cases). There was a negative correlation of the percentage of FoxP3+CD25+ cells with C-reactive protein (CRP) (r = -0.4), that of intracellular CD152+ with DAS28 (r = -0.35), erythrocyte sedimentation rate (ESR) (r = -0.46), and CRP (r=-0.54); that of FoxP3+CD127 with CRP (r = -0.42); that of CD25+CD127 with DAS28 (r = -0.38), Simplified Disease Activity Index (r = -0.41), Clinical Disease Activity Index (r = -0.36), ESR (r = -0.39), and CRP (r = -0.47) (p < 0.05 in all cases). Conclusion. The findings suggest that the functional activity of Treg is impaired in early RA, which has an impact on the activity of the inflammatory process.