Open AccessDetecção de 'CA. Phytoplasma phoenicium' em plantas de pervinca (Cathranthus roseus) em Uttar Pradesh, Índia
Author(s) -
N. N. Tiwari,
Ravindra Kumar Jain,
Ajay Tiwari
Publication year - 2022
Publication title -
bioscience journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.199
H-Index - 19
eISSN - 1981-3163
pISSN - 1516-3725
DOI - 10.14393/bj-v38n0a2022-56064
Subject(s) - phytoplasma , biology , nested polymerase chain reaction , broom , restriction fragment length polymorphism , amplicon , aster yellows , ornamental plant , botany , uttar pradesh , veterinary medicine , polymerase chain reaction , horticulture , genetics , ecology , gene , medicine , socioeconomics , sociology
Cathranthus roseus also known as periwinkle, an ornamental plant contains several medicinal values, was found with the symptoms of little leaf and witches’ broom at Shahjahanpur location with the incidence of up to 8%. The phytoplasma etiology was confirmed through scanning electron microscopy examination in all the four-leaf samples. Molecular analysis through PCR with universal primer pairs P1/P6 followed by nested PCR with R16F2n/R16r2 primers yielded ~1.2kbp amplicons in all the four symptomatic leaf samples. One amplicon was eluted, purified, sequenced, and used in BLASTn searches, which showed maximum identity of periwinkle isolate with several isolates of 16SrIX group of phytoplasma. Further, phylogenic analysis and in silico RFLP confirmed the association of 16SrIX-C subgroup phytoplasma in little leaf and witches broom plants which is the first report from India.