Open AccessAn efficient protocol for in vitro regeneration of peanut (Arachis hypogaea L.) cultivar L14
Author(s) -
Phùng Thị Bích Hòa,
Nguyen Hoang Tue,
Phan Thi Quyen Trang,
Lê Thị Thu Hằng,
Nguyễn Quang Đức Tiến,
Nguyễn Hoàng Lộc
Publication year - 2021
Publication title -
bioscience journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.199
H-Index - 19
eISSN - 1981-3163
pISSN - 1516-3725
DOI - 10.14393/bj-v37n0a2021-56949
Subject(s) - arachis hypogaea , explant culture , shoot , cultivar , biology , cotyledon , murashige and skoog medium , botany , horticulture , in vitro , biochemistry
The present work aims to establish an efficient protocol for in vitro regeneration of peanut (Arachis hypogaea) cultivar L14. The study showed that de-embryonated cotyledon was a suitable explant for shoot multiplication on MS medium containing 4 mg/L BAP. The highest number of shoots per explant obtained after 4 weeks of culture was up to 6.8 shoots. Shoots in vitro were able to produce a large number of approximately 11 roots on MS medium supplemented with 0.5 mg/L NAA. These results will be very useful in establishing an in vitro regeneration protocol for peanut cultivar L14 during gene transfer in the next studies to improve their disease resistance.