Open AccessDNA fragmentation rate in human thyroid tissue and carcinomas and its change under the influence of a-tocopherol and iodine ions in vitro
Author(s) -
T. M. Mishunina,
E. V. Kalinichenko,
Н Д Тронько,
T. M. Mishunina,
Ye. V. Kalinichenko
Publication year - 2009
Publication title -
problemy èndokrinologii
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.124
H-Index - 5
eISSN - 2308-1430
pISSN - 0375-9660
DOI - 10.14341/probl200955340-44
Subject(s) - dna fragmentation , fragmentation (computing) , thyroid , iodine , chemistry , thyroid carcinoma , dna , in vitro , follicular phase , endocrinology , medicine , microbiology and biotechnology , biochemistry , biology , apoptosis , programmed cell death , ecology , organic chemistry
The rate of DNA fragmentation was studied in intact thyroid tissue and thyroid carcinoma tissue. The determination of low molecular-weight DNA concentrations and the electrophoretic analysis of DNA isolated from the thyroid tissues and tumors, followed by the calculation of the content of oligonucleosome fractions, indicated a drastic reduction in the DNA fragmentation rate in papillary carcinomas and no its significant changes in follicular carcinomas. In the intact thyroid tissue, α-tocopherol and potassium iodide at a concentration of 10-7 M inhibited the rate of stimulated DNA fragmentation in vitro. The agents showed no effect in the papillary carcinoma tissue and their effect was similar in the follicular carcinoma tissue, but it was less pronounced in intact thyroid tissue.