Open AccessThe effect of chorionic gonadotropin on the functional activity of some populations of splenocytes
Author(s) -
С. В. Ширшев
Publication year - 1997
Publication title -
problemy èndokrinologii
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.124
H-Index - 5
eISSN - 2308-1430
pISSN - 0375-9660
DOI - 10.14341/probl199743640-42
Subject(s) - splenocyte , immunosuppression , human chorionic gonadotropin , chemistry , endocrinology , medicine , andrology , immunology , microbiology and biotechnology , antibody , biology , hormone
Selective immunomodulating effects of high (50 IU/ml) and low (10 IU/ml) doses of chorionic gonadotropin (CG) on the functional activity of A cells and intact splenic T and В lymphocytes were revealed for the syngeneic transfer system. One- hour incubation of nonfractionated splenocytes with CG suppressed the formation of antibody-producing cells (APC) in le- thally irradiated syngeneic recipients. Removal of macrophages from the suspension of intact splenocytes arrested CG-induced immunosuppression and, in case of a low dose, activated the formation of APC. Addition of CG in a low dose to cell culture rich in В lymphocytes activated APC formation. A high dose of the hormone exerted no immunostimulating effect of this kind but selectively suppressed the function of T lymphocytes fractionated by fdtration through nylon fiber filters. Study of the functional activity of A cells by luminol-dependent chemiluminescence showed CG in the studied doses to suppress the generation of active oxygen forms, thus determining the key role of A cells as inducers of CG-dependent immunosuppression in a heterogeneous suspension of splenocytes.