Open AccessThe effect of thyroxine isomers on the processes of free radical oxidation in the subcellular fractions of the rat cerebral cortex
Author(s) -
Olga Galkina,
В. М. Прокопенко,
F. E. Putilina,
N. D. Yeshchenko,
A. V. Arutyunyan
Publication year - 2000
Publication title -
problemy èndokrinologii
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.124
H-Index - 5
eISSN - 2308-1430
pISSN - 0375-9660
DOI - 10.14341/probl11862
Subject(s) - chemistry , chemiluminescence , in vitro , cerebral cortex , cortex (anatomy) , medicine , endocrinology , antioxidant , mitochondrion , fraction (chemistry) , phenol , hydrogen peroxide , hormone , biochemistry , chromatography , biology , organic chemistry , neuroscience
Effective concentrations for D and L thyroxin isomers were determined by the chemiluminescent (CL) method and their effects on free radical oxidation in the mitochondrial and synaptosomal fraction of adult rat cerebral cortex were studied in vitro. A OA DT4 in a model system with riboflavin was 2.2 times higher than L-T4. Effective concentrations for both thyroxin forms were 1$q= = 7.43 x 10~5+/-0.71 M for D-T4 and 15q=15.47 x 10~5+/1.23 M for L-T4. Thyroxin effect on membranous fraction of the brain cortex was studied in vitro using luminol-dependent peroxide CL. In normal concentrations (1 x 108 M) both hormone forms exerted equally intensive antioxidant effect which was more pronounced in the mitochondrial fraction, where CL decreased by 69 and 66%, while in the synaptosomal fraction it decreased only by 45 and 46%. Since D form possesses no hormonal activity, this effect may be due to phenol origin of thyroxin.