Open AccessHyperprolactinemia with the predominance of high-molecular immunoreactive prolactin: differences in the regulation of the levels of high-molecular and monomer forms in the blood
Author(s) -
А. А. Булатов,
Ye. Ye. Makarovskaya,
Ye. I. Marova,
Г. А. Мельниченко
Publication year - 1995
Publication title -
problemy èndokrinologii
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.124
H-Index - 5
eISSN - 2308-1430
pISSN - 0375-9660
DOI - 10.14341/probl11488
Subject(s) - prolactin , endocrinology , medicine , metoclopramide , dopaminergic , dopamine , stimulation , hormone , chemistry , monomer , molecular mass , secretion , biology , biochemistry , enzyme , organic chemistry , polymer , vomiting
The effect of drug stimulation of prolactin secretion on the level of its high-molecular (100 kD) and monomeric (23 kD) immunoreactive forms in the blood serum was studied in 5 women with idiopathic hyperprolactinemia with the predominance of the high-molecular form of the hormone. Metoclopramide, a dopamine receptor antagonist, was used for stimulation of prolactin secretion. Fractionation of blood serum immunoreactive prolactin was earned out using gel filtration. A single intravenous infusion of metoclopramide caused a rapid appreciable increase of the total level of immunoreactive hormone, mainly at the expense of its monomer fraction. In contrast to the monomer form, high-molecular prolactin poorly reacted to suppression of the dopaminergic tone. The time course of high- and low-molecular (monomer) forms’ response to metoclopramide differed. The results indicate differences in the regulation of the levels of high- and low-molecular immunoreactive forms of prolactin in the blood of patients with hyperprolactinemia with the predominance of high-molecular hormone and, hence, permit a hypothesis on a different biochemical nature of these two forms and on other than hypophyseal origin of the high-molecular form or its bulk.