Open AccessParaoxonase activity and lipid peroxidation in female patients with type 2 diabetes mellitus and without coronary heart disease
Author(s) -
M. Yu. Gorshunskaya
Publication year - 2003
Publication title -
problemy èndokrinologii
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.124
H-Index - 5
eISSN - 2308-1430
pISSN - 0375-9660
DOI - 10.14341/probl11394
Subject(s) - paraoxonase , medicine , lipid peroxidation , endocrinology , type 2 diabetes mellitus , aryldialkylphosphatase , antioxidant , coronary heart disease , diabetes mellitus , insulin resistance , chemistry , oxidative stress , pon1 , biochemistry , genotype , gene
The rate of lipid peroxidation and the parameters of antioxida- tive defense, including the activity of paraoxonase that is essential for the prevention of low-density lipoprotein oxidation, was studied in 229female patients with type 2 diabetes mellitus with and without coronary heart disease (CHD) under varying glycemic control. Carbohydrate and lipid metabolisms were explored by unified biochemical studies, blood insulin levels were measured by radioimmunological assay. The activity of paraoxonase associated with high-density lipoproteins of ester hydrolase was spectrophotometrically determined by using paraoxan as a substrate. Along with dyslipoproteinemia and insulin resistance, there was a drastically reduced paraoxonase activity that was associated with the high-density lipoproteins of the antioxidant enzyme and more pronounced in diabetics with CHD. A highly significant inverse correlation of the activity of the enzyme with the rate of lipid peroxidation and a less close relationship to basal glycemia have been verified, which substantiates the polygenic nature of decreased paraoxonase activity in diabetes mellitus.