Open AccessGenetic predictors of the effectiveness of the standard low-calorie diet in obese patients
Author(s) -
Т. Б. Сенцова,
Сенцова Татьяна Борисовна,
Olga Chernyak,
Черняк Ольга Олеговна,
I.V. Vorozhko,
Ворожко Илья Викторович,
K.M. Gapparova,
Гаппарова Камила Минкаиловна,
O. R. Grigoryan,
Григорьян Ольга Николаевна,
Yulya Chekhonina,
Чехонина Юлия Геннадьевна,
Alexandra Churycheva,
Чуричева Александра Михайловна
Publication year - 2016
Publication title -
ožirenie i metabolizm
Language(s) - English
Resource type - Journals
eISSN - 2306-5524
pISSN - 2071-8713
DOI - 10.14341/omet2016345-48
Subject(s) - apolipoprotein e , medicine , genotype , endocrinology , low calorie diet , turbidimetry , lipoprotein , cholesterol , obesity , triglyceride , high density lipoprotein , allele , biology , gene , genetics , biochemistry , weight loss , disease
Objectives: The aim of the study was to investigate the genetic predictors of standard low-calorie diet at different polymorphic variations ApoE and LPL genes in obese patients.Materials and methods: Our study involved 88 obese patients aged 19 to 67 years (27 men and 61 women, mean age 38.40 ± 2.82 years and the average BMI 41.71 ± 1.23). Study of lipid metabolism includes the determination of total cholesterol, triglycerides, low-density lipoprotein, high-density lipoprotein determined by spectrophotometry and immuno turbidimetry. Analysis of polymorphisms of LPL gene Ser447Ter (c.1595CG), Cys112Arg (c.388TC) and Arg158Cys (c.526CT) of ApoE gene was performed by polymerase chain reaction.Results: The results show significant differences in the efficacy of diet, depending on the genotype. We found that obese patients have significantly higher carrier genotypes c.388 T/T + c.526 C/C (by 20% reduced total cholesterol, by 19% – triglycerides, by20 % – low density lipoprotein, by 16% – high density lipoprotein, and by 9% – glucose), c.388 T/T + c.526 C/T ApoE gene (by 24% reduced total cholesterol, by 20% – triglycerides, and by 2% increased level of high-density lipoprotein) and C/C LPL gene (by 18% reduced total cholesterol concentration, by 17% – triglycerides, by 16% – high density lipoprotein, by 18% – low density lipoprotein, and by 9% – glucose).Conclusions: Thus, ApoE and LPL genes alleles can be used as biomarkers of prognosis of efficiency of a standard low-calorie diet therapy in obese patients.