Open AccessThe morphological characteristic of tissue repair in patients with neuropathic and neuroischemic forms of diabetic foot syndrome
Author(s) -
Ekaterina L. Zaitseva,
Л. П. Доронина,
R V Molchkov,
И А Воронкова,
Alla Y. Tokmakova
Publication year - 2015
Publication title -
saharnyj diabet
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.155
H-Index - 12
eISSN - 2072-0378
pISSN - 2072-0351
DOI - 10.14341/dm7587
Subject(s) - cd68 , granulation tissue , immunohistochemistry , medicine , osteopontin , pathology , cd31 , diabetes mellitus , extracellular matrix , diabetic foot , infiltration (hvac) , staining , wound healing , surgery , biology , endocrinology , physics , microbiology and biotechnology , thermodynamics
Aim. To evaluate the morphological and immunohistochemical features of granulation tissue formation in patients with diabetic foot syndrome.Materials and methods. We analysed the histological (light microscopy) and immunohistochemical (CD31, CD68, osteopontin, MMP-9 and TIMP-1) features of tissue repair processes in patients with diabetes mellitus. The study involved 63 patients with diabetic foot syndrome after surgical debridement.Results. We found severe intercellular oedema, poorly organised extracellular matrix, small amounts of fibroblast-like cells and expressed inflammatory infiltration, along with the presence of young granulation tissue. According to the results of the immunohistochemical studies, there were a moderate number of macrophages (immunopositive with antibodies to CD68), intense staining of MMP-9 and weak staining of TIMP-1 and osteopontin.Conclusion. According to the findings of the histological and immunohistochemical studies, tissue repair processes in patients with diabetes mellitus are decelerated.