Open AccessCoagulation factors II,V and methylene tetrahydrofolate reductase gene polymorphism in patients with diabetic nephropathy: prevalence, clinical and prognostic implications
Author(s) -
О. Ф. Сибирева,
Сибирева Ольга Филипповна,
Ekaterina Yur'evna Khitrinskaya,
Хитинская Екатерина Юрьевна,
V. V. Kalyuzhin,
Калюжин Вадим Витальевич,
A.E. Sazonov,
Сазонов Алексей Эдуардович,
И. И. Иванчук,
Иванчук Игорь Иванович,
Viktoriya Yur'evna Grankina,
Гранкина Виктория Юрьевна
Publication year - 2010
Publication title -
saharnyj diabet
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.155
H-Index - 12
eISSN - 2072-0378
pISSN - 2072-0351
DOI - 10.14341/2072-0351-6009
Subject(s) - methylenetetrahydrofolate reductase , medicine , reductase , genotype , gastroenterology , nephropathy , diabetic nephropathy , single nucleotide polymorphism , point mutation , gene mutation , diabetes mellitus , gene , endocrinology , mutation , genetics , enzyme , biology , biochemistry
Aim. To study prevalence, clinical and prognostic significance of prothrombotic genotypes pre-dominant in inborn thrombophilia in patients with diabeticnephropathy (DN). Materials and methods. A total of 90 patients with DN were examined; 54 and 36 cases suffered DM1 and DM2 respectively. Control group comprised100 healthy subjects. PCR was used to iden-tify single nucleotide substitution (C677T) in the methylene tetrahydrofolate reductase gene (MTHFR),point mutation in coagulation factor V gene (FV), and G202210A mutation in factor II gene (FII). Results. The probability of DN in patients with DM1 increases in the presence of Leiden mutation and in DM2 patients in the presence of single nucleotidesubstitu-tion (C677T) in MTHFR gene and G202210A mutation in the 3-untranslated region of FII. Conclusion. The prevalence of the above mutations associated with blood coagulation potential in DN patients is higher than in healthy subjects.Key words: diabetes mellitus, diabetic nephropathy, gene polymorphism, methylenetetrahydrofolate reductase