Open AccessUroven' visfatina syvorotki krovi i ekspressiyagena visfatina (PBEF1) v podkozhnoyi vistseral'noy zhirovoy tkani u detey
Author(s) -
А. В. Косыгина,
Sosunov,
V А Peterkova,
И И Дедов
Publication year - 2010
Publication title -
ožirenie i metabolizm
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.154
H-Index - 5
eISSN - 2306-5524
pISSN - 2071-8713
DOI - 10.14341/2071-8713-5083
Subject(s) - adipose tissue , overweight , medicine , subcutaneous adipose tissue , endocrinology , anthropometry , obesity
The aim of this study was to investigate the characteristics of visfatin - PBEF1 - gene expression in subcutaneous and visceraladipose tissue and serum levels of visfatin in children with relation to age and anthropometric parameters. The study included52 patients (27 boys (52%), 25 girls (48%) aged from 2,5 to 18 years (13,8 [7,4-15,1] years)), who underwent an elective surgical intervention.PBEF1 mRNA level was measured by real-time PCR and serum level of visfatin was quantified by immunoenzyme assay.According to our study visfatin serum concentration in children with normal body weight was 9,2 [7,4-11,6] ng/ml, whereas in theoverweight group - 9,5 [7,5-11,1] ng/ml (p=0,5). No statistically significant gender difference in serum visfatin levels was observed.No correlation between visfatin levels and age, pubertal stages and anthropometric indices in children was found. Statistically significantdifferences in the level of gene expression between subcutaneous and visceral adipose tissue were found (р=0,002) in thetotal group of children surveyed and children with normal weight, while there were no depot-specific differences in overweight children.The study did not reveal any dependence of PBEF1 expression on age and sex of children. Expression of PBEF1 in adiposetissue decreases with puberty (PBEF1VATγ=-0,24, р=0,02; PBEF1SATγ=-0,25, р=0,02). PBEF1 expression in adipose tissue wasnot correlated with the serum visfatin (R=-0,06, р=0,6).