Open AccesslncRNA Xist Regulates Osteoblast Differentiation by Sponging miR-19a-3p in Aging-induced Osteoporosis
Author(s) -
Shijie Chen,
Yuezhan Li,
Shuang Zhi,
Zhiyu Ding,
Yan Huang,
Weiguo Wang,
Ruping Zheng,
Haiyang Yu,
Jianlong Wang,
Minghua Hu,
Jun Miao,
Jinsong Li
Publication year - 2020
Publication title -
aging and disease
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.808
H-Index - 54
ISSN - 2152-5250
DOI - 10.14336/ad.2019.0724
Subject(s) - xist , adipogenesis , osteoblast , osteoporosis , mesenchymal stem cell , microbiology and biotechnology , cancer research , bone marrow , microrna , medicine , chemistry , endocrinology , biology , gene , in vitro , x inactivation , biochemistry , x chromosome
The switch between osteogenic and adipogenic differentiation of bone marrow mesenchymal stem cells (BMSCs) plays a key role in aging-induced osteoporosis. In this study, miR-19a-3p was obviously downregulated in BMSCs from aged humans and mice. Overexpressed miR-19a-3p evidently reduced aging-induced bone loss in mice and promoted osteogenic differentiation of BMSCs, while silenced miR-19a-3p manifestly increased aging-induced bone loss in mice and repressed osteogenic differentiation of BMSCs. Hoxa5 was significantly downregulated in the BMSCs from aged mice and contribute to miR-19a-3p-induced osteoblast differentiation as a direct target gene of miR-19a-3p. Furthermore, lncRNA Xist was found as a sponge of miR-19a-3p to repress BMSCs osteogenic differentiation. In conclusion, our study reveals the critical role of the lncRNA Xist/miR-19a-3p/Hoxa5 pathway in aging-induced osteogenic differentiation of BMSCs, indicating the potential therapeutic target for osteoporosis.