Open AccessNeuroprotective Effects of Endogenous Secretory Receptor for Advanced Glycation End-products in Brain Ischemia
Author(s) -
Shun Yu,
Ai Harashima,
Seiichi Munesue,
Masahiro Oishi,
Tsuyoshi Hattori,
Osamu Hori,
Yasuko Kitao,
Hiroshi Yamamoto,
taphat Leerach,
Mitsutoshi Nakada,
Yasuhiko Yamamoto,
Yasuhiko Hayashi
Publication year - 2020
Publication title -
aging and disease
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.808
H-Index - 54
ISSN - 2152-5250
DOI - 10.14336/ad.2019.0715
Subject(s) - rage (emotion) , ischemia , neuroprotection , glycation , receptor , medicine , endocrinology , brain ischemia , endogeny , pharmacology , microbiology and biotechnology , chemistry , neuroscience , biology
The receptor for advanced glycation end-products (RAGE) is expressed on human brain endothelial cells (HBEC) and is implicated in neuronal cell death after ischemia. We report that endogenous secretory RAGE (esRAGE) is a splicing variant form of RAGE that functions as a decoy against ischemia-induced neuronal cell damage. This study demonstrated that esRAGE was associated with heparan sulphate proteoglycans on HBEC. The parabiotic experiments between human esRAGE overexpressing transgenic (Tg), RAGE knockout (KO), and wild-type (WT) mice revealed a significant neuronal cell damage in the CA1 region of the WT side of parabiotic WT→WT mice, but not of Tg→WT mice, 7 days after bilateral common carotid artery occlusion. Human esRAGE was detected around the CA1 neurons in the WT side of the parabiotic Tg→WT pair, but not in the KO side of the Tg→KO pair. To elucidate the dynamic transfer of esRAGE into the brain, we used the blood-brain barrier (BBB) system (PharmaCo-Cell) with or without RAGE knockdown in endothelial cells. A RAGE-dependent transfer of esRAGE was demonstrated from the vascular to the brain side. These findings suggested that esRAGE is associated with heparan sulphate proteoglycans and is transferred into the brain via BBB to exert its neuroprotective effects in ischemia.