Open AccessCombining Injectable Plasma Scaffold with Mesenchymal Stem/Stromal Cells for Repairing Infarct Cavity after Ischemic Stroke
Author(s) -
Hongxia Zhang,
Feng-Yan Sun,
Jixian Wang,
Lin Xie,
Yi-Kan Cheng,
Mengxiong Pan,
Baiqi Shao,
GuoYuan Yang,
Shaohua Yang,
Qichuan Zhuge,
Kunlin Jin
Publication year - 2017
Publication title -
aging and disease
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.808
H-Index - 54
ISSN - 2152-5250
DOI - 10.14336/ad.2017.0305
Subject(s) - mesenchymal stem cell , scaffold , matrigel , stem cell , medicine , neural stem cell , transplantation , stromal cell , biomedical engineering , pathology , cancer research , microbiology and biotechnology , surgery , angiogenesis , biology
Stroke survivors are typically left with structural brain damage and associated functional impairment in the chronic phase of injury, for which few therapeutic options exist. We reported previously that transplantation of human embryonic stem cell (hESC)-derived neural stem cells together with Matrigel scaffolding into the brains of rats after focal ischemia reduced infarct volume and improved neurobehavioral performance. Matrigel is a gelatinous protein mixture extracted from mouse sarcoma cells, thus would not be approved for use as a scaffold clinically. In this study, we generated a gel-like scaffold from plasma that was controlled by changing the concentration of CaCl 2 . In vitro study confirmed that 10-20 mM CaCl 2 and 10-40% plasma did not affect the viability and proliferation of human and rat bone marrow mesenchymal stem/stromal cells (BMSCs) and neural stem cells (NSCs). We transplanted plasma scaffold in combination of BMSCs into the cystic cavity after focal cerebral ischemia, and found that the atrophy volume was dramatically reduced and motor function was significantly improved in the group transplanted with scaffold/BMSCs compared with the groups treated with vehicle, scaffold or BMSCs only. Our data suggest that plasma-derived scaffold in combination of BMSCs is feasible for tissue engineering approach for the stroke treatment.