Open AccessFUNCTIONALIZATION OF POLYMERIC NANOFIBERS USING PLATELETS FOR MELANOCYTE CULTURE
Author(s) -
Karolína Vocetková,
Věra Sovková,
Matěj Buzgo,
Radek Divín,
Evžen Amler,
Eva Filová
Publication year - 2020
Publication title -
lékař a technika
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.138
H-Index - 5
eISSN - 2336-5552
pISSN - 0301-5491
DOI - 10.14311/ctj.2020.1.03
Subject(s) - melanocyte , nanofiber , surface modification , tissue engineering , platelet , materials science , vitiligo , depigmentation , nanotechnology , microbiology and biotechnology , biomedical engineering , chemistry , biology , melanoma , medicine , immunology , cancer research , genetics
Tissue engineering is an interdisciplinary field that uses a combination of cells, suitable biomaterials and bioactive molecules to engineer the desired tissue and restore lost function. These principles have quickly begun to spread to the therapy of multiple diseases, including depigmentation disorders. The most common depigmentation disorder is vitiligo, a disease with deep psychosocial implications. Thanks to their unique properties, electrospun polymeric nanofibers represent a material suitable for tissue engineering applications. Furthermore, they may be functionalized with platelets, cells that contain a wide spectrum of growth factors and chemokines. The aim of this paper was to evaluate the functionalization of polymeric nanofibers with platelets and their effects in melanocyte culture. The scaffolds were visualized using scanning electron microscopy, the metabolic activity and proliferation of melanocytes was determined using MTS assay and dsDNA quantification, respectively. Furthermore, the melanocytes were stained and visualized using confocal microscopy. The acquired data showed that poly-ε-caprolactone functionalized with platelets promoted the viability and proliferation of melanocytes. According to the results, such a functionalized scaffold combining nanofibers and platelets may be suitable for melanocyte culture.