Open AccessMECHANICAL AND STRUCTURAL PROPERTIES OF COLLAGEN NANOFRIBROUS LAYERS UNDER SIMULATED BODY CONDITIONS
Author(s) -
Jitka Říhová,
Tomáš Suchý,
Lucie Vištějnová,
Lukáš Horný,
Monika Šupová
Publication year - 2019
Publication title -
acta polytechnica ctu proceedings
Language(s) - English
Resource type - Journals
ISSN - 2336-5382
DOI - 10.14311/app.2019.25.0073
Subject(s) - ultimate tensile strength , cell culture , chemistry , collagenase , molar ratio , biophysics , materials science , biochemistry , enzyme , composite material , biology , genetics , catalysis
The theme of this paper is the analysis of mechanical and structural properties of nanobrous COL under simulated body conditions and in the presence of osteoblasts and dermal broblasts. COL were prepared by electrostatic spinning of 8wt% collagen type I dispersion with 8wt% (to COL) of PEG in phosphate buer/ethanol solution (1/1vol). The stability of COL was enhanced by means of cross-linking with EDC and NHS at a molar ratio of 4:1. COL were exposed in culture medium for 21 days and human SAOS-2 human dermal broblasts and osteoblasts were cultured therein for 21 days as well. The cell culture on COL was assessed by uorescence microscopy and metabolic activity. Then the metabolic activity of both cell types grown on COL and PS were measured after 1, 7, 14 and 21 days using the Alamar Blue assay method. Mechanical properties were determined using an tensile test. The inuence of the cell activity on secondary structure of COL was veried by IR spectroscopy. Furthermore, the inuence of cells on COL was evaluated by SEM.