Open AccessPlasma MicroRNA Signature Validation for Early Detection of Colorectal Cancer
Author(s) -
Marta Herreros-Villanueva,
Saray Durán-Sanchón,
Ángel G. Martín,
Rosa Pérez-Palacios,
Elena Vila-Navarro,
María Marcuello,
Mireia Diaz-Centeno,
Joaquín Cubiella,
Maria Soledad Diez,
Luís Bujanda,
Ángel Lanas,
Rodrigo Jover,
Vicent Hernández,
Enrique Quintero,
Juan José Lozano,
Marta García-Cougil,
Ibon MartínezArranz,
Antoni Castells,
Meritxell Gironella,
Rocio Arroyo
Publication year - 2019
Publication title -
clinical and translational gastroenterology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.673
H-Index - 35
ISSN - 2155-384X
DOI - 10.14309/ctg.0000000000000003
Subject(s) - medicine , colorectal cancer , microrna , oncology , receiver operating characteristic , confidence interval , predictive value , asymptomatic , cancer , bioinformatics , gene , biology , biochemistry
OBJECTIVES: Specific microRNA (miRNA) signatures in biological fluids can facilitate earlier detection of the tumors being then minimally invasive diagnostic biomarkers. Circulating miRNAs have also emerged as promising diagnostic biomarkers for colorectal cancer (CRC) screening. In this study, we investigated the performance of a specific signature of miRNA in plasma samples to design a robust predictive model that can distinguish healthy individuals from those with CRC or advanced adenomas (AA) diseases. METHODS: Case control study of 297 patients from 8 Spanish centers including 100 healthy individuals, 101 diagnosed with AA, and 96 CRC cases. Quantitative real-time reverse transcription was used to quantify a signature of miRNA (miRNA19a, miRNA19b, miRNA15b, miRNA29a, miRNA335, and miRNA18a) in plasma samples. Binary classifiers (Support Vector Machine [SVM] linear, SVM radial, and SVM polynomial) were built for the best predictive model. RESULTS: Area under receiving operating characteristic curve of 0.92 (95% confidence interval 0.871–0.962) was obtained retrieving a model with a sensitivity of 0.85 and specificity of 0.90, positive predictive value of 0.94, and negative predictive value of 0.76 when advanced neoplasms (CRC and AA) were compared with healthy individuals. CONCLUSIONS: We identified and validated a signature of 6 miRNAs (miRNA19a, miRNA19b, miRNA15b, miRNA29a, miRNA335, and miRNA18a) as predictors that can differentiate significantly patients with CRC and AA from those who are healthy. However, large-scale validation studies in asymptomatic screening participants should be conducted.