Open AccessPropagação in vitro de Zingiber spectabile
Author(s) -
Michele Valquíria dos Reis,
Fernanda Carlota Nery,
Débora de Oliveira Prudente,
Patrícia Duarte de Oliveira Paiva,
Patrícia Duarte de Oliveira Paiva,
Rodrigo Therezan de Freitas,
Diogo Pedrosa Silva
Publication year - 2017
Publication title -
ornamental horticulture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.274
H-Index - 6
ISSN - 2447-536X
DOI - 10.14295/oh.v23i3.1035
Subject(s) - germination , activated charcoal , ornamental plant , imbibition , horticulture , sucrose , biology , plant propagation , botany , dry weight , cytokinin , chemistry , food science , auxin , gene , biochemistry , organic chemistry , adsorption
Zingiber spectabile is a tropical ornamental species with difficulties to obtain efficient propagation system. Thus, this study aimed to assess the in vitro propagation of Zingiber spectabile. Seed characterization was determined by measuring length, width and thickness, the weight of 1000 seeds and imbibition curve. In vitro germination of seeds was at constant (25 °C) or alternating temperatures (20-30 ºC). For optimization of in vitro multiplication, different concentrations of activated charcoal (0.0, 0.1 and 0.3%) and sucrose (0.0, 0.1, 0.3, 0.5 and 0.7 M) were evaluated. Plantlets were inoculated in flasks with different sealing systems (PVC covers with or without filters at the center) and culture media (MS or WPM). The plants were acclimatized in Plantmax® substrate. Seeds were of 6.06 mm length, 3.22 mm wide and 2.83 mm thick. The weight of 1,000 seeds corresponded to 46.4 g. The seed imbibition curve approaches to a tree phase pattern. Alternating temperatures induced high germination rates (68%). The addition of 0.3% activated charcoal provided higher root growth and plants with smaller number of senescent leaves. The best plant growth was obtained by the use of 0.1 M sucrose. All acclimatized plants survived (100%). The results demonstrate that Z. spectabile respond well to in vitro propagation.