Open AccessIndução de brotações in vitro de Dendrobium phalaenopsis Deang Suree em função do tempo de cultivo, luminosidade e BAP
Author(s) -
Yara Brito Chaim Jardim Rosa,
Cryslaine Cintia Alves dos Reis,
Jaqueline Clara Longo Casemiro,
Jackeline Schultz Soares,
José Carlos Sorgato,
Camila Soares Rosa Lemes
Publication year - 2015
Publication title -
ornamental horticulture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.274
H-Index - 6
ISSN - 2447-536X
DOI - 10.14295/oh.v21i3.621
Subject(s) - micropropagation , phalaenopsis , horticulture , dendrobium , biology , murashige and skoog medium , orchidaceae , botany , in vitro , tissue culture , biochemistry
The technique of in vitro micropropagation allows the production of large amounts of plants. However, some aspects of the orchid’s in vitro micropropagation are still poorly studied. This study analyzed the lighting conditions, time of cultivation and BAP concentrations on in vitro budburst of Dendrobium phalaenopsis Deang Suree. Therefore, a quantitative analysis of biometric parameters of plants grown during 90 and 180 days on ½ MS medium under different lighting conditions (18.90 mol m-2 s-1, 14.85 mol m-2 s-1, 9.45 mol m-2 s-1) and concentrations of BAP (0; 0.5; 1; 1.5; 2; 2.5 e 3 mg L-1). The best results of budburst were obtained during 180 days, under white fluorescent light (18.90 mol m-2 s-1) and using 1.5±0.1 mg L-1 of BAP.