Open AccessThree species of Colletotrichum are associated with anthracnose of pomegranate in Brazil
Author(s) -
Janaíne Rossane Araújo Silva-Cabral,
Lourdes Regina Lopes Batista,
Jaqueline Figueredo de Oliveira Costa,
Edna Peixoto da Rocha Amorim,
Gaus Silvestre de Andrade Lima,
Iraíldes Pereira Assunção
Publication year - 2020
Publication title -
comunicata scientiae
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.238
H-Index - 12
eISSN - 2177-5133
pISSN - 2176-9079
DOI - 10.14295/cs.v11i0.3126
Subject(s) - colletotrichum , biology , conidium , phylogenetic tree , punica , mycelium , botany , colletotrichum gloeosporioides , horticulture , gene , genetics
Pomegranate is a fruit rich in vitamins and secondary metabolites used in traditional medicine and industry. However, production losses have been associated the anthracnose, disease caused by Colletotrichum species. This is an important disease of the pomegranate, as it affects the yield and the quality of the fruits. The present study aimed to investigate which species of Colletotrichum are associated with anthracnose disease in pomegranate in the Northeast region of Brazil, using multi-locus phylogenetic analysis and morpho-cultural characteristics. The total DNA extracted was amplified with GAPDH, TUB2, CAL, ACT genes and the ITS-rDNA region. The sequences obtained were used for the construction of phylogenetic trees of Bayesian inference. The mycelial growth rate, size and shape of the conidia and appressories were evaluated for the morpho-cultural characterization of the species. Six isolates were analysis and three species belonging to the C. gloeosporioides complex were identified in this study. This is the first report of C. theobromicola (2) in pomegranate fruit in Brazil and C. siamense (2) and C. fructicola (2) in the world.