Open AccessCulture and molecular analysis of Enterococcus faecalis and antimicrobial susceptibility of clinical isolates from patients with failure endodontic treatment
Author(s) -
Marcos Sérgio Endo,
Fernanda Graziela Corrêa Signoretti,
Vivian Sayuri Kitayama,
Ariane Cássia Salustiano Marinho,
Frederico Canato Martinho,
Brenda Paula Figueiredo de Almeida Gomes
Publication year - 2014
Publication title -
brazilian dental science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.153
H-Index - 6
ISSN - 2178-6011
DOI - 10.14295/bds.2014.v17i3.1016
Subject(s) - enterococcus faecalis , moxifloxacin , microbiology and biotechnology , amoxicillin , benzylpenicillin , penicillin , ampicillin , antibiotics , clavulanic acid , vancomycin , erythromycin , antimicrobial , rifampicin , biology , bacteria , staphylococcus aureus , genetics
Objective: The aim of this study was to evaluate the minimal inhibitory concentrations (MIC) of different antibiotic agents against to the most prevalent microorganism found in root-filled canals by culture and molecular approaches. Material and Methods: The microbial samples were taken either from thirty root-filled canals after removal of gutta-percha. Culture methods and 16s rDNA assay were used to identify the E faecails present in the samples. The antimicrobial susceptibilities of the isolates of E faecalis were determined by MIC values using the E test System and interpreted according to the Clinical and Laboratory Standards Institute guidelines. The following antibiotics were used: benzylpenicillin, amoxicillin, amoxicillin-clavulanic acid, erythromycin, azithromycin, vancomycin, chloramphenicol, tetracycline, doxycycline, ciprofloxacin, rifampicin and moxifloxacin. Results: E faecalis were isolated (7/30) and detected (13/30) by culture and PCR assay, respectively. All tested E faecalis (n=12) were highly sensitive to amoxicillin, moxifloxacin, vancomycin, benzylpenicillin and amoxicillin-clavulanic acid. Some antibiotics were resistant against E faecalis strains such as rifampicin (4/12), tetracycline (2/12), doxycycline (1/12), erythromycin (3/12) and azythromycin (8/12). Conclusion: Amoxicillin, amoxicillin-clavulanic acid, benzylpenicillin, vancomycin and moxifloxacin were the most active antibiotics, in vitro, against E faecalis clinical strains, with all the isolates being susceptible. Azithromycin and erythromycin were least effective, with none percentage of isolates being susceptible, during laboratory testing. Moreover, E faecalis were identified more frequently by PCR assay than by culture technique.Keywords: Retreatment; Antibiotics, antimicrobial susceptibility; Enterococcus faecalis; Antibiotic resistance.