Open AccessPreliminary results of studying the genetic diversity of Malaxis monophyllos (Orchidaceae) in the Amur region
Author(s) -
Elena Terentieva,
Galina V. Degtjareva,
С.В. Ефимов,
Т.И. Варлыгина
Publication year - 2021
Publication title -
problemy botaniki ûžnoj sibiri i mongolii
Language(s) - English
Resource type - Journals
eISSN - 2686-9268
pISSN - 2313-3929
DOI - 10.14258/pbssm.2021085
Subject(s) - herbarium , orchidaceae , biology , phylogenetic tree , internal transcribed spacer , clade , genetic diversity , botany , nucleotide diversity , ribosomal dna , nuclear dna , evolutionary biology , mitochondrial dna , genetics , gene , genotype , population , demography , sociology , haplotype
For research the plants of Malaxis monophyllos (L.) Sw. were sampled in the Amur region and takenfrom the herbarium collections of the same region (MW and MHA). The plants of M. monophyllos have a high degreeof vari-ability of quantitative morphological characters. First, we paid attention to the number of leaves, the height ofplants and peduncles, the number and size of leaves and the number of flowers. Therefore, the purpose of this workwas compara-tive molecular studies of individuals of the same species with different morphological characteristics.Internal transcribed spacers (ITS1 and ITS2) of the 18S–26S region of nuclear ribosomal DNA were chosen as amolecular marker. On the molecular phylogenetic tree, the analyzed M. monophyllos plants form clade with a highposterior probability (1.0). The ITS sequences of the analyzed samples are not identical. The level of geneticdifferentiation of nucleotide sequences was 0.12 %, which corresponds to the variability within the species, whichconfirms the belonging of the analyzed samples to the same species. Since the species has a large area, it is necessary toanalyze plant samples from other regions to obtain reliable results. The different regions of the nuclear and plastidgenomes should be added to the genetic analysis.