Open AccessAJUGA TURKESTANICA ALTERNATIVE SOURCE FOR EKDISTEN PRODUCTION
Author(s) -
А. У. Маматханов,
Temurbek Atakhanovich Hajibaev,
Р. М. Халилов
Publication year - 2022
Publication title -
himiâ rastitelʹnogo syrʹâ
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.2
H-Index - 6
eISSN - 1029-5151
pISSN - 1029-5143
DOI - 10.14258/jcprm.2022019271
Subject(s) - extraction (chemistry) , chromatography , ethyl acetate , chemistry , column chromatography , methanol , acetone , chloroform , raw material , sorbent , organic chemistry , adsorption
The process of ecdysteron extraction from the aerial part of Ajuga turkestanica was studied. On the basis research results established the following extraction conditions: extractant – 95% ethyl alcohol, the particle size of the raw material is 2–5 mm and the process temperature at 20–30 °C. Proposed fivefold ecdysteron extraction from the aerial part of Ajuga turkestanica. To remove hydrophobic impurities from the concentrated and water-diluted extract, proposed was a sequential treatment with extraction benzine and ethyl acetate. From the purified aqueous solution, ecdysteron is extracted with n-butanol. From a concentrated butanol solution, precipitation is recommended of iridoids from acetone. For chromatographic purification, the simultaneous use of aluminum oxide and silica gel in the ratio of the sum of ecdysteroids – sorbent 1 : 20. The quality as eluent mix selected propanol-2 – chloroform in a volumetric ratio of 1 : 3, and for crystallization a mixture of methanol with ethyl acetate in a volume ratio of 1 : 9. The technology has been developed for obtaining the ecdisten substance from the arial part of Ajuga turkestanica.