Open AccessQUANTITATIVE DETERMINATION OF ARTEANNUIN B – A SESQUITERPENE LACTONE FROM ARTEMISIA ANNUA
Author(s) -
R. F. Мukhamatkhanova,
V. V. Uzbekov,
Dilnoza Karimberdievna Mutalova,
Munirakhon Akhmatkhon Kizi Mamatkhanova,
I. D. Sham’yanov,
Р. М. Халилов
Publication year - 2021
Publication title -
himiâ rastitelʹnogo syrʹâ
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.2
H-Index - 6
eISSN - 1029-5151
pISSN - 1029-5143
DOI - 10.14258/jcprm.2021038476
Subject(s) - artemisia annua , sesquiterpene lactone , chromatography , repeatability , chemistry , herb , high performance liquid chromatography , artemisinin , sesquiterpene , stereochemistry , traditional medicine , medicinal herbs , biology , medicine , plasmodium falciparum , malaria , immunology
The HPLC (High performance liquid chromatography) method for the determination of arteannuin B in the herb Artemisia annua has been developed. The method is suitable for its validation characteristics, such as specificity parameters (convergence of the retention times of artianuin B in the standard and sample), suitability of the chromatographic system (separation efficiency), linearity (R2 = 0.99929 at y = 9.38x + 245.98), range of application (from 50 to 150%), accuracy in terms of repeatability (RSD = 3.45%) and correctness (Z = 100.36 ± 0.56%), determined experimentally, are within the recommended values. As a marker compound, it was proposed to use a standard sample of arteannuin B. For this, a standard sample of arteannuin B was obtained with a purity of at least 99%, which is confirmed IR, 1HNMR, 13CNMR spectral data. Organoleptic and physicochemical parameters were determined. It has been established that the presence of impurities in an amount of not more than 1% is allowed in a standard sample of arteannuin B. It was established that the content of arteannuin B in the raw material should be at least 0.2%. The development and validation HPLC-UV method for the determination of arteannuin B have to used to standardization the herb of Artemisia annua.