Open AccessANTIOXIDANT ACTIVITY OF TRITERPENE GLYCOSIDE (CORTUSOSIDE A) FROM CORTUSA MATTHIOLI L. PLANT
Author(s) -
Бешлей Игорь Васильевич,
Безматерных Ксения Викторовна,
Ширшова Татьяна Ивановна,
Володин Владимир Витальевич,
Смирнова Галина Васильевна
Publication year - 2020
Publication title -
himiâ rastitelʹnogo syrʹâ
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.2
H-Index - 6
eISSN - 1029-5151
pISSN - 1029-5143
DOI - 10.14258/jcprm.2020037416
Subject(s) - chemistry , chelation , triterpene , dpph , antioxidant , ascorbic acid , glycoside , radical , stereochemistry , catechin , organic chemistry , polyphenol , food science , medicine , alternative medicine , pathology
The antioxidant activity of triterpene glycoside, first isolated from the aboveground part of the plant Cortusa matthioli L. and identified as β-D-xylopyranosyl-(1→2)-β-D-glucopyranosyl-(1→4)-[β-D-glucopyranosyl-(1→2)]-α-L-arabinopyranoside-(1→3)-13β,28-epoxyolean-30-al-3β,16α-diol (cortusoside A), is studied. Tests for the ability of cortusoside A to bind 2,2-diphenyl-1-picrylhydrazyl (DPPH) radicals did not reveal any activity of this compound. However, in experiments to study the ability to chelate Fe2+ ions, its sufficiently high iron chelating activity was found, which was only 2.24 times lower compared to the powerful Fe2+ chelator dipyridyl. The EC50 values for cortusoside A and dipyridyl were 0.417±0.057 and 0.186±0.018 mM, respectively. Literature analysis has shown that the structural analogue of cortusoside A, saxifragifolin B, has a much weaker iron chelating ability (13,4 times) compared to the standard Fe2+ EDTA-Na2 ion chelator, as well as a weak ability to bind free radicals of DPPH compared to the reference antioxidants – catechin and ascorbic acid (50 and 32 times, respectively). Despite the structural identity of the molecules cortusoside A and saxifragifolin B, low radiculopathy activity cortusosoide A may be due to differences in the structure of these substances (optical or geometric isomerism), as well as different methods were used in its definition.