Open AccessPOLYSACCHARIDES OF THREE SAUSSUREA DC SPECIES (S. CONTROVERSA, S. SALICIFOLIA, S. FROLOVII): ISOLATION, CHARACTERIZATION AND INFLUENCE ON NO-PRODUCING PROPERTIES OF MACROPHAGES
Author(s) -
Yaroslav Reshetov,
Anastasiya Aleksandrovna Ligacheva,
Yelena Yur'yevna Avdeeva,
М. Г. Данилец,
Victoria V. Golovchenko,
Yevgeniya Sergeyevna Trofimova,
Yekaterina Igorevna Gulina,
Yevgeniy Yur'yevich Sherstoboev,
A. M. Guryev,
К. И. Ровкина,
Sergey Vladimirovich Krivoshchekov,
М. В. Белоусов
Publication year - 2019
Publication title -
himiâ rastitelʹnogo syrʹâ
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.2
H-Index - 6
eISSN - 1029-5151
pISSN - 1029-5143
DOI - 10.14258/jcprm.2019045483
Subject(s) - rhamnose , polysaccharide , arabinose , galactose , mannose , monosaccharide , chemistry , xylose , sugar , carrageenan , biochemistry , biology , fermentation
Polysaccharides PS1 and PS2 with a yield 1–2% were obtained from the above-ground part of three species of Saussurea: S. controversa DC., S. salicifolia L. and S. frolovii Ledeb. sequential extraction with water at 25 and 70 °C. PS1 free from protein impurities. A protein that is not removed by the Sevag method is co-extracted with PS2. All polysaccharides contain residues of uronic acids, the highest content found in PS1 and PS2 from S. contrоversa. The molecular weights (Mw) of PS1 from S. controversa, S. salicifolia and S. frolovii were 448.13, 158.49, 64.03 kDa and PS2 – 101.82, 94.60, 225.42 kDa, respectively. Interspecific differences in the monosaccharide composition of polysaccharides were revealed. Galactose (Gal) and Arabinose (Ara) residues are major, and Rhamnose (Rha), Xylose (Xyl) and Mannose (Man) residues are minor components of the carbohydrate chains isolated by PS. PS1 S. salicifolia and S. frolovii and PS2 S. salicifolia do not contain endotoxins impurities and have a NO-activating effect on antigen-presenting cells (macrophages), significantly exceeding the effect of muramyl dipeptide.