Open AccessTHE DEVELOPMENT AND VALIDATION OF THE METHOD TO QUANTIFY THE AMOUNT OF PHENOLIC ACIDS IN AERIAL PARTS OF ZIZIPHORA CLINOPODIOIDES LAM.
Author(s) -
Андреева Валерия Юрьевна,
Калинкина Галина Ильинична,
Ли Валерия Владимировна
Publication year - 2019
Publication title -
himiâ rastitelʹnogo syrʹâ
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.2
H-Index - 6
eISSN - 1029-5151
pISSN - 1029-5143
DOI - 10.14258/jcprm.2019034683
Subject(s) - chemistry , chlorogenic acid , chromatography , extraction (chemistry)
Development and validation of method for quantitative determination of the amount of phenolic acids in the aerial part of the Ziziphora clinopodioides Lam.
Objective: to develop and conduct a validation assessment of the method for determining the content of the amount of phenolic acids in the Ziziphora clinopodioides.
The method of quantification of phenolic acids equivalent to chlorogenic acid by direct spectrophotometry in aerial parts of the Ziziphora clinopodioides has been developed based on the study of the absorption spectrum of an aqueous-alcoholic extract. The influence of the following factors on the yield of phenolic acids was studied: the nature of the extractant, the ratio of raw material: extractant, the degree of particle fineness, the extraction multiplicity, the extraction temperature; their optimum values were established.
The method of quantitative determination of the amount of phenolic acids in the aerial part of the Ziziphora clinopodioides is validated; it has been established that the proposed method is highly sensitive, correct, reproducible and suitable for use in an analytical laboratory based on the data obtained.