STUDY OF THE POSSIBILITY OF USING IR-SPECTROSCOPY FOR THE IDENTIFICATION OF A VARIETY OF SEA BUCKTHORN FRUIT (НIPPOPHAES RHAMNOIDES L.)

The purpose of this study was to study the feasibility of using IR spectroscopy (IRS) to identify the fruit of sea buckthorn and buckthorn varieties. Fourier species of sea-buckthorn of buckthorn species studied were analyzed by FT-IR spectroscopy of disturbed total internal reflection (FTIR). IR spectra of sea buckthorn fruits was characterized by the presence of specific absorption bands: for grades «Gallery», «Вotanical», «Trofimovskaya» and «Krasnokarminovaya» in the range 3009-3006; 1456-1443 and 1379-1375 cm-1; for varieties «Rowan», «Botanical amateur» and «Student» in the range of 1338-1321 cm-1; for varieties «Rowan», «Botanical aromatic» and «Krasnokarminovaya» in the range of 1155-1146 cm-1; for varieties «Rowan» and «Krasnokarminovaya» at 914 cm-1; for varieties «Galerit», «Botanicheskaya» and «Krasnokarminovaya» in the range of 609-592 cm-1; for varieties «Trofimovskaya» and «Krasnokarminovaya» at 814-812 cm-1; for the variety «Rowan» at 424 cm-1 and for the variety «Trofimovskaya» at 771 cm-1. The highest intensity in absorption maxima is characteristic for infrared spectra of the fruits of the varieties «Rowan», «Botanical Amateur», «Botanical Aromatic» and «Student». Identified specific frequency in the spectrum can be regarded as characteristic for a particular grade and use them as markers in determining the authenticity and graded supplies dry fruit of sea buckthorn by IR spectroscopy. The formation of hydrogen bonds and their characteristics (size and energy) between molecules of biologically active substances (BAS) in the LRS studied are established. The revealed intermolecular hydrogen bonds belong to the type of strong bonds, since their energy exceeds 5 kcal/mol, and the length is about 2.7 Ǻ. Polyphenolic BAS in fruits, according to the IRS, form polyassociates with characteristic absorption frequencies at 3400-3200 cm-1.