Open AccessTHE RESEARCH OF THE SPECTRUM OF BIOLOGICALLY ACTIVE FLAVONOIDS GRASS HYPERICUM PERFORA-TUM L. FLORA OF THE REPUBLIC OF TATARSTAN BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY
Author(s) -
Л. З. Хуснетдинова,
А. Н. Акулов,
Светлана Алексеевна Дубровная
Publication year - 2017
Publication title -
himiâ rastitelʹnogo syrʹâ
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.2
H-Index - 6
eISSN - 1029-5151
pISSN - 1029-5143
DOI - 10.14258/jcprm.2017041841
Subject(s) - hypericum perforatum , hypericum , rutin , hyperforin , herb , flavonoid , quercetin , botany , traditional medicine , hypericin , high performance liquid chromatography , chemistry , biology , medicinal herbs , chromatography , medicine , pharmacology , organic chemistry , antioxidant
The article presents the results of the study of the dependence of the flavonoid content of the herb Hypericum perforatum L., from growth conditions of cenopopulations in various ecological, cenotic conditions of the Republic of Tatarstan. Plant biocenosis were considered – meadows, Border of broadleaf and pine forest or artificial planting of pine. A qualitative and quantitative analysis of flavonoids in the herb of Hypericum perforatum was carried out by high-performance liquid chromatography. In studying water-alcohol extracts of H. perforatum L. flavonoids – rutin, quercetin, bisapigenin were identified by HPLC analysis. It is shown that the ecological, cenotic conditions of growth of Hypericum perforatum affect the quantitative content of phenolic compounds. It has been established that in investigated cenopopulations in the territory of the Republic of Tatarstan, rutin is the dominant flavonoid in medicinal plant raw materials. Maximum amount of this compound detected in the samples of Hypericum perforatum confined to the pine biocenosis of Tukaevsky and Drozhzhanovsky districts under significant quantity level of quercetin. In all variants of the experiment, bisapiginin was detected in small amounts. Plants of Hypericum perforatum, grow in different plant biocenosis differed in the various contents of the compounds of the phenolic complex, which is probably due to the ecological, cenotic and climatic conditions of growth.