Open AccessFlavonoids from Alpinia officinarum as Potential Anti-Tubercular Agents Using Molecular Docking Studies
Author(s) -
Varsha S. Honmore,
Arun D. Natu,
Vijay M. Khedkar,
Dhiman Sarkar,
Supada R. Rojatkar
Publication year - 2022
Publication title -
asian journal of chemistry/asian journal of chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.145
H-Index - 34
eISSN - 0975-427X
pISSN - 0970-7077
DOI - 10.14233/ajchem.2022.23620
Subject(s) - galangin , chemistry , in vivo , traditional medicine , pharmacology , docking (animal) , in vitro , flavonoid , kaempferol , biochemistry , biology , antioxidant , medicine , microbiology and biotechnology , nursing
Bioguided isolation yielded three flavonoids galangin (1), kaempferide (2) and kaempferol (3) frommethanol extract of Alpinia officinarum Hance. Galangin exhibited highest antitubercular activity,with MIC of < 4.0 μg/mL against M. bovis BCG (in vitro) and M. tuberculosis (ex vivo), thankaempferide. In vitro and ex vivo macrophage infection model assay revealed the inhibition of bothactive and dormant stage for M. tuberculosis H37Ra and M. bovis BCG on exposure to galangin.Molecular docking studies into the active site of DprE1 enzyme helped to understand the ligand-proteininteractions and establish a structural basis for inhibition of M. tuberculosis. These flavonoids indicatedtheir non-specificity towards M. tuberculosis by testing against Gram-positive and Gram-negativebacteria and also least cytotoxic up to 100 μg/mL on three human cancer cell lines THP-1, PANC-1and A549, respectively. So these flavonoids are inhibitors against M. tuberculosis that can be exploredfurther as potential antitubercular drugs.