Open AccessGC-MS Analysis, Molecular Docking and Hepatoprotective Effect of Ethanolic Extract of Capparis zeylanica on CCl4-Induced Hepatotoxicity in Rats
Author(s) -
Raghunandan Hota,
B. K. Nanda,
Biswaranjan Behera,
Anindya Bose,
Debajyoti Das
Publication year - 2022
Publication title -
asian journal of chemistry/asian journal of chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.145
H-Index - 34
eISSN - 0975-427X
pISSN - 0970-7077
DOI - 10.14233/ajchem.2022.23616
Subject(s) - chemistry , gallic acid , rutin , traditional medicine , lipid peroxidation , quercetin , antioxidant , pharmacology , dpph , biochemistry , medicine
Capparis zeylanica (Capparaceae) is a climbing shrub, commonly known as ‘Asadua’ in Oriya language.Folkloric it is used as anti-inflammatory, anti-rheumatic, hepatitis and liver tonics. The present workaimed to investigate the hepatoprotective activity of Capparis zeylanica root. The ethanolic extractwas subjected to GC-MS analysis and quantification of rutin, quercetin and gallic acid by HPLC. Invitro antioxidant study was evaluated by DPPH, nitric oxide radical and hydrogen peroxide assay.Hepatoprotective activity was performed by CCl4 (1.25 ml/kg, I.P) induced Wistar albino rats at doseof 200 and 300 mg/kg using silymarin (100 mg/kg) as standard. The liver tissue homogenate wasexamined for lipid peroxidation, SOD, CAT and GSH assay. Serum was taken for biochemical analysisand liver for histopathological study. In silico molecular docking study was performed by the indentifiedcompounds. Gallic acid was quantified as highest in HPLC analysis. Fifty compounds were identifiedby GC-MS analysis. The extract exhibited significant free radical scavenging effect at highestconcentration. The level of liver function enzymes (AST, ALT and ALP), bilirubin and protein weresignificantly improved after the administration of 200 and 300 mg/kg of extract. Alterations in SOD,CAT, GSH and lipid peroxidation levels were significantly checked by extract. Compounds propane,1, 1-dipropoxy- (-5.2); cis-9-hexadecenal (-6.0); 17-octadecen-14-yn-1-ol (-6.0);6-butyl-1,4-cycloheptadiene (-6.5) exhibited greater docking score as compared to standard silymarin(-5.1). The hepatoprotective potential of C. zeylanica could be due to its antioxidant effect and thesynergistic effect of these compounds.