Open AccessSynthesis of Gold Nanoparticles using Endophytic streptomyces and its Bactericidal Activities against Enteric Bacterial Pathogens
Author(s) -
S. Farook Basha,
Periyasamy Sivalingam,
Manickam Muthuselvam
Publication year - 2022
Publication title -
asian journal of chemistry/asian journal of chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.145
H-Index - 34
eISSN - 0975-427X
pISSN - 0970-7077
DOI - 10.14233/ajchem.2022.23587
Subject(s) - chemistry , fourier transform infrared spectroscopy , dynamic light scattering , nuclear chemistry , zeta potential , colloidal gold , surface plasmon resonance , spectroscopy , antibacterial activity , ultraviolet visible spectroscopy , nanoparticle , bacteria , nanotechnology , materials science , chemical engineering , organic chemistry , biology , physics , quantum mechanics , engineering , genetics
The present study aimed to report the rapid and one-step synthesis of gold nanoparticles (AuNPs) using the cell-free supernatant (CFS) ofan endophytic Streptomyces sp. BDUSMP S05 isolated from Solanum trilobatum. The synthesized AuNPs were characterized by usingUV-visible spectroscopy (UV-vis), Fourier-transform infrared spectroscopy (FTIR), energy dispersive X-ray (EDAX) spectroscopy andX-ray diffraction (XRD). The UV-visible spectroscopy (UV-vis) absorption of colloidal AuNPs displayed a surface plasma resonance(SPR) centred at 533 nm. FESEM micrograph analysis showed that synthesized particles were predominantly spherical with a 20-30 nmsize range. Dynamic light scattering (DLS) analysis showed that the average particle size varied between 2-28 nm. Zeta potential analysisindicated the negative value of -35.2 mV characteristic for stable AuNPs. The synthesized AuNPs displayed significant antimicrobialactivity against Gram-negative bacteria, including Eescherichia coli, Salmonella typhi and Vibrio cholerae compared to CFS alone. Theresults revealed that endophytic Streptomyces sp. potential to be used for the synthesis of antibacterial gold nanobased materials.