Open AccessGraphene Oxide Nanoparticles Decorated Pencil Lead as Urea Sensing Electrode
Author(s) -
Saravjeet Singh,
Minakshi Sharma,
Geeta Singh
Publication year - 2021
Publication title -
asian journal of chemistry/asian journal of chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.145
H-Index - 34
eISSN - 0975-427X
pISSN - 0970-7077
DOI - 10.14233/ajchem.2021.23501
Subject(s) - electrode , graphene , chemistry , urea , scanning electron microscope , urease , biosensor , nanocomposite , electrochemistry , nuclear chemistry , oxide , nanoparticle , chemical engineering , analytical chemistry (journal) , nanotechnology , materials science , chromatography , composite material , organic chemistry , biochemistry , engineering
In present study, a low-cost and steady electrochemical biosensor has been developed based on grapheneoxide nanoparticles (GONPs), electrodeposited on the pencil lead electrode (PLE). The physical andmorphological studies revealed the nano-scale range of GONPs that has an average grain size/layerthickness of 2.27 nm and agglomeration size of 90-120 nm. Urease enzyme was immobilized onPLE/GONPs electrode after surface treatment with 1-ethyl-3-(3-dimethylaminopropyl)carbodiimidehydrochloride (EDC)/N-hydroxysuccinimide (NHS) to impart stable peptide bond. Scanning electronmicroscopy (SEM) studies demonstrate the difference between unmodified and modified PLE electrodesand depict the spherical, circular and flaky-like morphology. The electrochemical analysis of ureaconsiderably improved on the PLE/GONPs/Ur compared to the untailored PLE electrode. The reportedurea biosensor operated in a linear dynamic range between 0.30-50 mM that attained a recognition limitof 0.06 mM and exhibited an advanced exposure sensitivity of 0.814 μA mM-1 cm-2.