Open AccessChromatographic Estimation of Water Soluble Vitamins in Wild Edible Plants
Author(s) -
Tapan Seal,
Kausik Chaudhuri,
Basundhara Pillai
Publication year - 2021
Publication title -
asian journal of chemistry/asian journal of chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.145
H-Index - 34
eISSN - 0975-427X
pISSN - 0970-7077
DOI - 10.14233/ajchem.2021.23452
Subject(s) - chemistry , chromatography , vitamin , gradient elution , detection limit , acetic acid , high performance liquid chromatography , elution , food science , acetonitrile , biochemistry
A reversed-phase high performance liquid chromatographic technique has been developed for thesimultaneous quantitation of water soluble vitamins in 10 potent wild edible plants consumed by thetribal people of North-eastern region in India. The chromatographic separations of vitamins wereassessed on Acclaim C18 column using a mobile phase of acetonitrile and aqueous trifluoro aceticacid solution with gradient elution. The experimental results exhibited that for different plants, thevitamin C content ranged between 0.15 ± 0.003 to 8.10 ± 0.03 mg/100g dry plant material (DPM).The vitamin B2 content was determined high in C. album (2.64 ± 0.03 mg/100g DPM) and significantamount of vitamin B9 (1.44 ± 0.03 mg/100g) was detected in E. acuminata. The results showed thatthese plants are rich sources of vitamins, which can contribute immensely to nutrition and food security.The high percentage of recovery and low limit of detection confirm the suitability of the method forsimultaneous estimation of vitamins in these 10 wild edible plants.