Open AccessAntibacterial Activity of Borassus flabellifer Vinegar-Graphene Quantum Dots Against Gram-Positive and Gram-Negative Bacteria
Author(s) -
Amnuay Noypha,
Paweena Porrawatkul,
gyao Teppaya,
Parintip Rattanaburi,
Saksit Chanthai,
Prawit Nuengmatcha
Publication year - 2021
Publication title -
asian journal of chemistry/asian journal of chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.145
H-Index - 34
eISSN - 0975-427X
pISSN - 0970-7077
DOI - 10.14233/ajchem.2021.23376
Subject(s) - chemistry , staphylococcus aureus , antibacterial activity , escherichia coli , minimum bactericidal concentration , nuclear chemistry , gram positive bacteria , minimum inhibitory concentration , bacteria , gram negative bacteria , graphene , microbiology and biotechnology , antimicrobial , biochemistry , nanotechnology , organic chemistry , biology , materials science , genetics , gene
Borassus flabellifer vinegar–graphene quantum dots (BFV-GQDs) were successfully synthesized usinga pyrolysis method with Borassus flabellifer vinegar (BFV) as the precursor. All the samples werecharacterized using ultraviolet-visible spectrophotometry (UV-Vis), scanning electron microscopy(SEM) and energy-dispersive X-ray spectroscopy (EDX). The antibacterial activities of BFV-GQDsagainst strains of Gram-negative bacteria (Escherichia coli) and Gram-positive bacteria (Staphylococcusaureus) were determined using the agar well diffusion method for preliminary screening, whileminimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) weredetermined using the broth macro-dilution method. The zones of inhibition were compared with thoseof citric acid–graphene quantum dots (CA-GQDs). It was observed that the synthesized BFV-GQDsdemonstrated excellent antibacterial activity against Staphylococcus aureus (82.3%) and goodantibacterial activity against Escherichia coli (73.3%). The MIC of BFV-GQDs against E. coli was6.25 mg/mL and S. aureus was 12.5 mg/mL, whereas the MBC of BFV-GQDs against E. coli was12.5 mg/mL and S. aureus was 25.0 mg/mL.